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3T3‐L1 cells and perivascular adipocytes are not equivalent in amine transporter expression
Author(s) -
Ismail Alex,
AyalaLopez Nadia,
Ahmad Maleeha,
Watts Stephanie W.
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12513
Subject(s) - transporter , chemistry , 3t3 l1 , microbiology and biotechnology , expression (computer science) , biophysics , biochemistry , adipocyte , biology , adipose tissue , gene , computer science , programming language
Rat perivascular adipose tissue ( PVAT ) stores, takes up, and releases norepinephrine ( NE ; Ayala‐Lopez et al . (2014) Pharmacol Res Perspect 2, e00041). We hypothesized that 3T3‐L1 adipocytes would exhibit similar behaviors and, thus, could serve as a model for PVAT adipocytes. However, basal levels of NE were not detected in 3T3‐L1 adipocytes. While incubation of 3T3‐L1 adipocytes with exogenous NE increased their cellular NE content, the mRNA expression of several NE transporters [e.g., norepinephrine transporter ( NET )] were not detected in these cells. Similarly, we observed expression of the vesicular monoamine transporter 1 ( VMAT 1) in 3T3‐L1 adipocytes by qRT ‐ PCR and immunostaining, but stimulation of the cells with tyramine (100 μ m ) did not cause a significant release of NE . These studies support that 3T3‐L1 adipocytes are not an adequate model of perivascular adipocytes for studying NE handling.