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Functional identification of At AVT 3 , a family of vacuolar amino acid transporters, in Arabidopsis
Author(s) -
Fujiki Yuki,
Teshima Hiromitsu,
Kashiwao Shinji,
KawanoKawada Miyuki,
Ohsumi Yoshinori,
Kakinuma Yoshimi,
Sekito Takayuki
Publication year - 2017
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12507
Subject(s) - vacuole , arabidopsis , amino acid , cytosol , biochemistry , yeast , saccharomyces cerevisiae , heterologous expression , biology , arabidopsis thaliana , transporter , gene , microbiology and biotechnology , chemistry , cytoplasm , enzyme , recombinant dna , mutant
Amino acids stored in the vacuoles are exported to the cytosol mainly for protein synthesis; however, the molecular identity of vacuolar amino acid exporters remains obscure in plants. Here, we demonstrate that the heterologous expression of At AVT 3 genes, Arabidopsis homologs of AVT 3 and AVT 4 encoding vacuolar amino acid exporters in yeast, reduces vacuolar amino acid levels in the avt3 ∆ avt4 ∆ yeast cells. In vitro experiments revealed that 14 C‐labeled Ala and Pro are exported from vacuolar membrane vesicles by AtAvt3A in an ATP ‐dependent manner. In Arabidopsis , AtAvt3A fused with green fluorescent protein localizes to the vacuolar membrane. We propose that At AVT 3 family represents the long sought‐for vacuolar amino acid exporters in plants.