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Selenoprotein P neutralizes lipopolysaccharide and participates in hepatic cell endoplasmic reticulum stress response
Author(s) -
Zhao Yongzhong,
Banerjee Shuvojit,
Huang Ping,
Wang Xinning,
Gladson Candece L.,
Heston Warren D.,
Foster Charles B.
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12494
Subject(s) - endoplasmic reticulum , selenoprotein p , lipopolysaccharide , unfolded protein response , sepsis , chemistry , hepatocyte , microbiology and biotechnology , biology , biochemistry , immunology , oxidative stress , catalase , glutathione peroxidase , in vitro
Low serum selenium or selenoprotein P (Se PP ) levels have been repetitively observed in severe sepsis. The role of Se PP in sepsis is incompletely characterized. To test the hypothesis that lipopolysaccharide ( LPS ) interacts with Se PP , we investigated the interaction between LPS and the histidine‐rich (His‐rich) regions of Se PP . We demonstrate that both purified Se PP and synthetic peptides corresponding to the His‐rich motifs neutralized LPS . In addition, we used a hepatocyte model to study the fate of Se PP in response to LPS or endoplasmic reticulum ( ER ) stress. Our findings indicate that ER stress increases the cellular level of Se PP and promotes its nuclear localization.