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Identification of a classic nuclear localization signal at the N terminus that regulates the subcellular localization of Rbfox2 isoforms during differentiation of NM u MG and P19 cells
Author(s) -
Wenzel Manuel,
Schüle Martin,
Casanovas Sonia,
Strand Dennis,
Strand Susanne,
Winter Jennifer
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12492
Subject(s) - subcellular localization , nuclear localization sequence , gene isoform , microbiology and biotechnology , nls , identification (biology) , n terminus , chemistry , biology , nucleus , biochemistry , gene , peptide sequence , cytoplasm , botany
Nuclear localization of the alternative splicing factor Rbfox2 is achieved by a C‐terminal nuclear localization signal ( NLS ) which can be excluded from some Rbfox2 isoforms by alternative splicing. While this predicts nuclear and cytoplasmic localization, Rbfox2 is exclusively nuclear in some cell types. Here, we identify a second NLS in the N terminus of Rbfox2 isoform 1A that is not included in Rbfox2 isoform 1F. Rbfox2 1A isoforms lacking the C‐terminal NLS are nuclear, whereas equivalent 1F isoforms are cytoplasmic. A shift in Rbfox2 expression toward cytoplasmic 1F isoforms occurs during epithelial to mesenchymal transition ( EMT ) and could be important in regulating the activity and function of Rbfox2.