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Identification of HLA ‐ DRB 1*09:01‐restricted Mycobacterium tuberculosis CD 4 + T‐cell epitopes
Author(s) -
Liu SuDong,
Zhang ShiMeng,
Wang Hui,
He JianChun,
Yang XiaoFan,
Du XiaLin,
Ma Li
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12478
Subject(s) - epitope , mycobacterium tuberculosis , peripheral blood mononuclear cell , antigen , human leukocyte antigen , t cell , tuberculosis , biology , tumor necrosis factor alpha , immunology , microbiology and biotechnology , virology , immune system , medicine , in vitro , biochemistry , pathology
CD 4 + T cells play an essential role in protection against Mycobacterium tuberculosis ( MTB ) infection. We identified three HLA ‐ DRB 1*09:01‐restricted CD 4 + T‐cell epitopes derived from the dominant secreted MTB antigens 38 kDa (Rv3804c) and Ag85A (Rv0934). The antigens were screened for epitopes by in silico prediction programs and analysis of IFN ‐γ induction in the peripheral blood mononuclear cells ( PBMC s) from TB patients. In response to three of the high‐affinity predicted epitopes derived from 38 kDa and Ag85A, CD 4 + T cells from HLA ‐ DRB 1*09:01 TB patients were stimulated to produce IFN ‐γ and Tumor Necrosis Factor (TNF)‐α. The three epitopes were also found to induce the proliferation of CD 4 + T cells by carboxyfluorescein succinimidyl ester‐diluted assays. These HLA ‐ DRB 1*09:01‐restricted CD 4 + T‐cell epitopes facilitate analysis of the role of 38 kDa‐ and Ag85A‐specific T cells in MTB infection and pave way for the design of vaccines against tuberculosis.