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Human DNA polymerase α interacts with mismatch repair proteins MSH 2 and MSH 6
Author(s) -
Itkonen Harri M.,
Kantelinen Jukka,
Vaara Markku,
Parkkinen Sinikka,
Schlott Bernhard,
Grosse Frank,
Nyström Minna,
Syväoja Juhani E.,
Pospiech Helmut
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12475
Subject(s) - dna , polymerase , chemistry , microbiology and biotechnology , biochemistry , biology
High fidelity of genome duplication is ensured by cooperation of polymerase proofreading and mismatch repair (MMR) activities. Here, we show that human mismatch recognizing proteins MutS homolog 2 ( MSH 2) and MSH 6 copurify and interact with replicative Pol α. This enzyme also is the replicative primase and replicates DNA with poor fidelity. We show that MSH 2 associates with known human replication origins with different dynamics than DNA polymerase (Pol α). Furthermore, we explored the potential functional role of Pol α in the mismatch repair reaction using an in vitro mismatch repair assay and observed that Pol α promotes mismatch repair. Taken together, we show that human Pol α interacts with MSH 2‐ MSH 6 complex and propose that this interaction occurs during the mismatch repair reaction.