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Carbon monoxide shifts energetic metabolism from glycolysis to oxidative phosphorylation in endothelial cells
Author(s) -
Kaczara Patrycja,
Motterlini Roberto,
Kus Kamil,
Zakrzewska Agnieszka,
Abramov Andrey Y.,
Chlopicki Stefan
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12434
Subject(s) - oxidative phosphorylation , glycolysis , bioenergetics , mitochondrion , microbiology and biotechnology , adenosine triphosphate , chemistry , metabolism , respiration , biochemistry , phosphorylation , cellular respiration , biology , botany
Carbon monoxide ( CO ) modulates mitochondrial respiration, but the mechanisms involved are not completely understood. The aim of the present study was to investigate the acute effects of CO on bioenergetics and metabolism in intact EA .hy926 endothelial cells using live cell imaging techniques. Our findings indicate that CORM ‐401, a compound that liberates CO , reduces ATP production from glycolysis, and induces a mild mitochondrial depolarization. In addition, CO from CORM ‐401 increases mitochondrial calcium and activates complexes I and II . The subsequent increase in mitochondrial respiration leads to ATP production through oxidative phosphorylation. Thus, our results show that nonactivated endothelial cells rely primarily on glycolysis, but in the presence of CO , mitochondrial Ca 2+ increases and activates respiration that shifts the metabolism of endothelial cells from glycolysis‐ to oxidative phosphorylation‐dependent ATP production.