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TEX 13 is a novel male germ cell‐specific nuclear protein potentially involved in transcriptional repression
Author(s) -
Kwon Jun Tae,
Jin Sora,
Choi Heejin,
Kim Jihye,
Jeong Juri,
Kim Jaehwan,
Cho Chunghee
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12433
Subject(s) - biology , germ cell , spermatogenesis , microbiology and biotechnology , sertoli cell , psychological repression , gene , nuclear protein , regulation of gene expression , transcriptional regulation , gene expression , cell , genetics , transcription factor , endocrinology
The identification and characterization of male germ cell‐specific genes is crucial to understanding the mechanisms of male germ cell development. In this study, we investigated the protein encoded by the novel mouse germ cell‐specific gene testis‐expressed gene 13 ( Tex13 ). We found that TEX 13 expression is testis‐ and germ cell‐specific and is regulated in a stage‐specific manner via translational repression. Immunostaining of testicular cells and sperm showed that TEX 13 is localized in the nuclei of spermatogenic cells and the redundant nuclear envelope of mature sperm. Remarkably, we found that TEX 13 possesses transcriptional repressor activity and that its overexpression in GC ‐2 cells altered the expression levels of 130 genes. Our results suggest that TEX 13 has a potential role in transcriptional regulation during spermatogenesis.