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Loss of Msp1p in Schizosaccharomyces pombe induces a ROS ‐dependent nuclear mutator phenotype that affects mitochondrial fission genes
Author(s) -
Delerue Thomas,
Khosrobakhsh Farnoosh,
Daloyau Marlène,
Emorine Laurent Jean,
Dedieu Adrien,
Herbert Christopher J.,
Bonnefoy Nathalie,
ArnaunéPelloquin Laetitia,
Belenguer Pascale
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12432
Subject(s) - mitochondrial fission , schizosaccharomyces pombe , mitochondrial fusion , mitochondrial dna , biology , mitochondrion , microbiology and biotechnology , schizosaccharomyces , mfn2 , atp–adp translocase , phenotype , genetics , dnaja3 , nuclear dna , nuclear gene , gene , inner mitochondrial membrane , saccharomyces cerevisiae
Mitochondria continually fuse and divide to dynamically adapt to changes in metabolism and stress. Mitochondrial dynamics are also required for mitochondrial DNA (mt DNA ) integrity; however, the underlying reason is not known. In this study, we examined the link between mitochondrial fusion and mt DNA maintenance in Schizosaccharomyces pombe , which cannot survive without mt DNA , by screening for suppressors of the lethality induced by loss of the dynamin‐related large GTP ase Msp1p. Our findings reveal that inactivation of Msp1p induces a ROS ‐dependent nuclear mutator phenotype that affects mitochondrial fission genes involved in suppressing mitochondrial fragmentation and mt DNA depletion. This indicates that mitochondrial fusion is crucial for maintaining the integrity of both mitochondrial and nuclear genetic information. Furthermore, our study suggests that the primary roles of Msp1p are to organize mitochondrial membranes, thus making them competent for fusion, and maintain the integrity of mt DNA .