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Alternative splicing of the receptor‐like kinase Nt‐Sd‐ RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?
Author(s) -
Sanabria Natasha M.,
Dubery Ian A.
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12428
Subject(s) - alternative splicing , biology , rna splicing , protein kinase domain , kinase , microbiology and biotechnology , gene , genetics , exon , mutant , rna
Nt‐Sd‐ RLK encodes an S‐domain lectin receptor‐like kinase that is induced in response to microbe‐associated molecular pattern molecules ( MAMP s) such as lipopolysaccharide ( LPS ). In this study, we investigated the alternative splicing of Nt‐Sd‐ RLK in response to LPS stimulation. Our data indicate that in nonstimulated cells, a shorter transcript of Nt‐Sd‐ RLK is generated and that in response to LPS , alternative splicing produces the full‐length transcript. We propose that the extracellular domain of Nt‐Sd‐ RLK encoded by the shorter transcript functions in pathogen surveillance. Once this domain binds LPS , alternative splicing generates the kinase domain‐containing Nt‐Sd‐ RLK that activates downstream signalling leading to a defence response. Thus, our findings suggest that plant defence signalling may be regulated through the alternative splicing of receptor‐like kinases involved in pathogen recognition.