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Overexpression of Os MYB 1R1– VP 64 fusion protein increases grain yield in rice by delaying flowering time
Author(s) -
Wang Jiachang,
Wu Fuqing,
Zhu Shanshan,
Xu Yang,
Cheng Zhijun,
Wang Jiulin,
Li Chaonan,
Sheng Peike,
Zhang Huan,
Cai Maohong,
Guo Xiuping,
Zhang Xin,
Wang Chunming,
Wan Jianmin
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12374
Subject(s) - myb , fusion protein , biology , activator (genetics) , gene , transcription factor , genetics , recombinant dna
VP 64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an Os MYB 1R1– VP 64 fusion protein that enhanced the grain yield of rice cultivar ‘Kita‐ake’ by 35%. Os MYB 1R1 – VP 64 regulated grain yield of transgenic plants mainly by extending the vegetative growth stage. Further analysis indicated that Os MYB 1R1 – VP 64 delayed flowering by down‐regulating expression of Heading date 3a ( Hd3a ) and RICE FLOWERING LOCUST 1 ( RFT 1 ) through repression of Early heading date 1 ( Ehd1 ). The expression pattern of Os MYB 1R1 was constitutive and rhythmically controlled. Os MYB 1R1 protein had transcriptional activation activity and was localized to the nucleus. Our findings suggest that the Os MYB 1R1 – VP 64 construct can be used to increase grain yield in rice.