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ATG 15 encodes a phospholipase and is transcriptionally regulated by YAP 1 in Saccharomyces cerevisiae
Author(s) -
Ramya Visvanathan,
Rajasekharan Ram
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12369
Subject(s) - saccharomyces cerevisiae , biology , phospholipase , biochemistry , phosphatidylserine , phospholipase a1 , serine , mutant , phospholipase c , microbiology and biotechnology , enzyme , gene , phospholipid , membrane
Phospholipases play a vital role in maintaining membrane phospholipids. In this study, we found that deletion of the three major phospholipases B in Saccharomyces cerevisiae did not affect the hydrolysis of phospholipids, thus suggesting the presence of other, as yet unidentified, phospholipases. Indeed, in silico analysis of the S. cerevisiae genome identified 13 proteins that contain a conserved, putative serine hydrolase motif. In addition, expression profiling revealed that ATG 15 (Autophagy 15) was highly expressed in the phospholipase B triple mutant. ATG 15 encodes a phospholipase that preferentially hydrolyzes phosphatidylserine. Our analysis of the ATG 15 promoter identified binding sites for Yap1p. In vivo and in vitro results showed that Yap1p positively regulates ATG 15 expression. Collectively, we demonstrate that Atg15p is a phosphatidylserine lipase and that Yap1p activates the expression of ATG 15 during autophagy.