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N‐glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier
Author(s) -
Guan Ziqiang,
Delago Antonia,
Nußbaum Phillip,
Meyer Benjamin,
Albers SonjaVerena,
Eichler Jerry
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12341
Subject(s) - sulfolobus acidocaldarius , dolichol , sulfolobus , glycosylation , biochemistry , glycoprotein , archaea , chemistry , biology , biosynthesis , enzyme , gene
N‐glycosylation is a post‐translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius , glycoproteins are modified by an N‐linked tribranched hexasaccharide reminiscent of the N‐glycans assembled in Eukarya. Previously, hexose‐bearing dolichol phosphate was detected in a S. acidocaldarius Bligh–Dyer lipid extract. Here, we used a specialized protocol for extracting lipid‐linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N‐glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N‐glycosylation.