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Mitochondrial transit peptide exhibits cell penetration ability and efficiently delivers macromolecules to mitochondria
Author(s) -
Jain Aastha,
Chugh Archana
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12329
Subject(s) - mitochondrion , microbiology and biotechnology , hela , cell penetrating peptide , cell , macromolecule , peptide , chemistry , transfection , biophysics , biology , biochemistry , gene
Mitochondrial malfunction under various circumstances can lead to a variety of disorders. Effective targeting of macromolecules (drugs) is important for restoration of mitochondrial function and treatment of related disorders. We have designed a novel cell‐penetrating mitochondrial transit peptide (Cp MTP ) for delivery of macromolecules to mitochondria. Comparison between properties of cell‐penetrating peptides ( CPP s) and mitochondrial signal sequences enabled prediction of peptides with dual ability for cellular translocation and mitochondrial localization. Among the predicted peptides, Cp MTP translocates across HeLa cells and shows successful delivery of noncovalently conjugated cargo molecules to mitochondria. Cp MTP may have applications in transduction and transfection of mitochondria for therapeutics.