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γ‐Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae
Author(s) -
Tatehashi Yuki,
Watanabe Daisuke,
Takagi Hiroshi
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12318
Subject(s) - saccharomyces cerevisiae , autophagy , biochemistry , yeast , biology , protease , protein kinase a , ubiquitin , protein biosynthesis , ribosome , microbiology and biotechnology , biosynthesis , gene , enzyme , chemistry , rna , apoptosis
γ‐Glutamyl kinase ( GK ; the PRO 1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild‐type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO 1 genetically interacts with UBP 3 , which encodes ubiquitin‐specific protease, and is required for selective autophagy of ribosomes (ribophagy). Interestingly, yeast cells with PRO 1 deletion or expressing inactive GK display a defect for ribophagy but not for nonselective autophagy, indicating that GK activity is indispensable for ribophagy. Gene disruption analysis suggests that ribophagy is important for cell survival during nitrogen starvation.