Premium
Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development
Author(s) -
Kim In Jung,
Kim Sangsoo,
Park Jeahyun,
Eom Intae,
Kim Sunam,
Kim JinHong,
Ha Sung Chul,
Kim Yeon Gil,
Hwang Kwang Yeon,
Nam Ki Hyun
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12316
Subject(s) - metal ions in aqueous solution , metal , biosensor , chemistry , ion , materials science , nanotechnology , inorganic chemistry , organic chemistry
Many fluorescent proteins ( FP s) show fluorescence quenching by specific metal ions, which can be applied towards metal biosensor development. In this study, we investigated the significant fluorescence quenching of Dronpa by Co 2+ and Cu 2+ ions. Crystal structures of Co 2+ ‐, Ni 2+ ‐ and Cu 2+ ‐bound Dronpa revealed previously unseen, unique, metal‐binding sites for fluorescence quenching. These metal ions commonly interact with surface‐exposed histidine residues (His194‐His210 and His210‐His212), and interact indirectly with chromophores. Structural analysis of the Co 2+ ‐ and Cu 2+ ‐ binding sites of Dronpa provides insight into FP ‐based metal biosensor engineering.