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Enzymatic production of oroxylin A and hispidulin using a liverwort flavone 6‐ O ‐methyltransferase
Author(s) -
Zhang YuYing,
Xu RuiXue,
Gao Shuai,
Cheng AiXia
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12312
Subject(s) - baicalein , chemistry , methyltransferase , flavonoid , enzyme , o methyltransferase , biochemistry , pharmacology , biology , gene , antioxidant , methylation
Oroxylin A and hispidulin, compounds which are abundant in both Scutellaria and liverwort species, are important lead compounds for the treatment of ischemic cerebrovascular disease. Their enzymatic synthesis requires an O ‐methyltransferase able to interact with the related flavonoid's 6‐ OH group, but such an enzyme has yet to be identified in plants. Here, the gene encoding an O ‐methyltransferase (designated PaF6 OMT ) was isolated from the liverwort species Plagiochasma appendiculatum . A test of alternative substrates revealed that its strongest preferences were baicalein and scutellarein, which were converted into, respectively, oroxylin A and hispidulin. Allowed a sufficient reaction time, the conversion rate of these two substrates was, respectively, 90% and 100%. PaF6 OMT offers an enzymatic route to the synthesis of oroxylin A and hispidulin.