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Reduced levels of Dusp3/Vhr phosphatase impair normal spindle bipolarity in an Erk1/2 activity‐dependent manner
Author(s) -
Tambe Mahesh Balasaheb,
Narvi Elli,
Kallio Marko
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12310
Subject(s) - dual specificity phosphatase , phosphatase , mitosis , kinase , microbiology and biotechnology , downregulation and upregulation , mitogen activated protein kinase , ectopic expression , mapk/erk pathway , biology , chemistry , phosphorylation , biochemistry , gene
Dual specificity phosphatase‐3 (Dusp3/Vhr) regulates cell cycle progression by counteracting the effects of mitogen‐activated protein kinases (Mapk) Erk1/2 and Jnk. Despite the known upregulation of Dusp3 at M phase in mammalian cells, its mitotic functions are poorly characterized. Here, we report that loss of Dusp3 by RNA i leads to the formation of multipolar spindles in human mitotic cancer cells in an Erk1/2‐dependent manner. In the phosphatase‐silenced cells, the normal bipolar spindle structure was restored by chemical inhibition of Erk1/2 and ectopic overexpression of Dusp3. We propose that at M phase Dusp3 keeps Erk1/2 activity in check to facilitate normal mitosis.