Premium
Soy‐ghretropin, a novel ghrelin‐releasing peptide derived from soy protein
Author(s) -
Nakato Junya,
Aoki Hayato,
Iwakura Hiroshi,
Suzuki Hideyuki,
Kanamoto Ryuhei,
Ohinata Kousaku
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12306
Subject(s) - ghrelin , orexigenic , soy protein , chemistry , peptide , endocrinology , medicine , peptide hormone , secretion , hormone , biochemistry , biology , neuropeptide , receptor , neuropeptide y receptor
We investigated exogenous secretagogues of ghrelin, which is an orexigenic hormone isolated from the stomach. We found that the tryptic digest of soy β‐conglycinin stimulated ghrelin secretion by the ghrelin‐producing cell line, MGN 3‐1. We then identified a 22‐amino acid peptide corresponding to the β‐conglycinin α‐subunit(192–213) [β CG α(192–213)] from an active fraction separated by HPLC . The N‐terminal undecapeptide of β CG α(192–213), NKNPFLFGSNR , exhibited ghrelin‐releasing activity at a lower dose than that of β CG α(192–213). We named NKNPFLFGSNR ‘soy‐ghretropin’, which corresponds to β CG α(192–202). Neither [des‐N 1 K 2 ]‐soy‐ghretropin nor [des‐R 11 ]‐soy‐ghretropin stimulated ghrelin secretion; hence, both the N‐ and C‐terminal structures of soy‐ghretropin were indispensable. Orally administered soy‐ghretropin increased plasma ghrelin levels and food intake in vivo . Soy‐ghretropin is the first exogenous ghrelin‐releasing peptide derived from food protein.