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Origin of bimodal fluorescence enhancement factors of Chlorobaculum tepidum reaction centers on silver island films
Author(s) -
Maćkowski Sebastian,
Czechowski Nikodem,
Ashraf Khuram U.,
Szalkowski Marcin,
Lokstein Heiko,
Cogdell Richard J.,
Kowalska Dorota
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12292
Subject(s) - fluorescence , plasmon , excitation wavelength , excitation , absorption (acoustics) , context (archaeology) , materials science , wavelength , photochemistry , molecular physics , bacteriochlorophyll , chemical physics , absorption spectroscopy , chemistry , optoelectronics , optics , physics , biology , paleontology , organic chemistry , quantum mechanics , composite material , pigment
We focus on the spectral dependence of plasmon‐induced enhancement of fluorescence of Chlorobaculum tepidum reaction centers. When deposited on silver island film, they exhibit up to a 60‐fold increase in fluorescence. The dependence of enhancement factors on the excitation wavelength is not correlated with the absorption spectrum of the plasmonic structure. In particular, the presence of one (or multiple) trimers of the Fenna–Matthews–Olson ( FMO ) protein reveals itself in bimodal distribution of enhancement factors for the excitation at 589 nm, the wavelength corresponding to bacteriochlorophyll absorption of FMO and the core of the RC . We conclude that the structure of multichromophoric complexes can substantially affect the impact of plasmonic excitations, which is important in the context of assembling functional biohybrid systems.