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Depletion of autophagy receptor p62/ SQSTM 1 enhances the efficiency of gene delivery in mammalian cells
Author(s) -
Tsuchiya Megumi,
Ogawa Hidesato,
Koujin Takako,
Kobayashi Shouhei,
Mori Chie,
Hiraoka Yasushi,
Haraguchi Tokuko
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12262
Subject(s) - transfection , gene knockdown , embryonic stem cell , reporter gene , microbiology and biotechnology , gene delivery , hela , hek 293 cells , gene knockout , ectopic expression , gene , chemistry , biology , cell culture , gene expression , biochemistry , genetics
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/ SQSTM 1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62‐knockout murine embryonic fibroblast ( MEF ) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, si RNA ‐mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem ( mES ) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.