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Identification and characterization of a bacterial hyaluronidase and its production in recombinant form
Author(s) -
Messina Luciano,
Gavira Jose A.,
Pernagallo Salvatore,
UncitiBroceta Juan D.,
Sanchez Martin Rosario M.,
DiazMochon Juan J.,
Vaccaro Susanna,
ConejeroMuriel Mayte,
PinedaMolina Estela,
Caruso Salvatore,
Musumeci Luca,
Di Pasquale Roberta,
Pontillo Angela,
Sincinelli Francesca,
Pavan Mauro,
Secchieri Cynthia
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12258
Subject(s) - hyaluronidase , recombinant dna , bacteria , biochemistry , chemistry , substrate (aquarium) , isolation (microbiology) , cloning (programming) , enzyme , hyaluronic acid , extracellular , microbiology and biotechnology , biology , gene , ecology , genetics , programming language , computer science
Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial‐scale recombinant production, purification and full characterization, including X‐ray structure determination at 1.45 Å, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis . The recombinant S. koganeiensis Hyal ( rH yal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rH yal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross‐infection.