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Identification of a bipartite nuclear localization signal in the silkworm Masc protein
Author(s) -
Sugano Yudai,
Kokusho Ryuhei,
Ueda Masamichi,
Fujimoto Masaru,
Tsutsumi Nobuhiro,
Shimada Toru,
Kiuchi Takashi,
Katsuma Susumu
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12246
Subject(s) - bipartite graph , identification (biology) , nuclear localization sequence , signal (programming language) , computational biology , chemistry , microbiology and biotechnology , biology , computer science , botany , theoretical computer science , nucleus , graph , programming language
The silkworm Masculinizer ( Masc ) gene encodes a CCCH ‐tandem zinc finger protein that controls both masculinization and dosage compensation. Masc protein is a nuclear protein, but the mechanism underlying the transport of this protein into the nucleus has not yet been elucidated. Here, we identified a functional bipartite nuclear localization signal ( NLS ) located between residues 274 and 290 of the Masc protein. Sequence comparison revealed that this bipartite NLS is evolutionarily conserved in Masc proteins from other lepidopteran insects. Furthermore, we showed that the degree of nuclear localization is not associated with the masculinizing activity of the Masc protein.