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Identification of putative Z‐ring‐associated proteins, involved in cell division in human pathogenic bacteria Helicobacter pylori
Author(s) -
Kamran Mohammad,
Sinha Swati,
Dubey Priyanka,
Lynn Andrew M.,
Dhar Suman K.
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12230
Subject(s) - ftsz , cell division , helicobacter pylori , biology , cell , bacteria , nucleoid , bacterial cell structure , microbiology and biotechnology , complementation , ring (chemistry) , phenotype , genetics , chemistry , gene , escherichia coli , organic chemistry
Cell division in bacteria is initiated by FtsZ, which forms a Z ring at the middle of the cell, between the nucleoids. The Z ring is stabilized by Z ring‐associated proteins (Zaps), which crosslink the FtsZ filaments and provide strength. The deletion of Zaps leads to the elongation phenotype with an abnormal Z ring. The components of cell division in Helicobacter pylori are similar to other gram negative bacteria except for the absence of few components including Zaps. Here, we used HHsearch to identify homologs of the missing cell division proteins and got potential hits for ZapA and ZapB, as well as for few other cell division proteins. We further validated the function of the putative ZapA homolog by genetic complementation, immuno‐colocalization and biochemical analysis.