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Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGIα‐dependent pathway
Author(s) -
Piwkowska Agnieszka,
Rogacka Dorota,
Audzeyenka Irena,
Kasztan Małgorzata,
Angielski Stefan,
Jankowski Maciej
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12228
Subject(s) - microbiology and biotechnology , calcium in biology , endoplasmic reticulum , medicine , intracellular , podocyte , endocrinology , chemistry , plasma membrane ca2+ atpase , calcium , calcium signaling , signal transduction , biology , atpase , biochemistry , kidney , enzyme , proteinuria
Podocytes are dynamic polarized cells that lie on the surface of glomerular capillaries and comprise an essential component of the glomerular filtration barrier. Insulin provoked a sustained, approximately 70%, increase in intracellular calcium concentration in podocytes. RT‐PCR revealed the presence of mRNA encoding sarco/endoplasmic reticulum calcium ATPase isoforms 1–3, and plasma membrane Ca 2+ pump (PMCA) isoforms 1,3,4; mRNA levels were depressed by the addition of insulin. Inhibitors of PMCA, and the Na + ‐Ca 2+ exchanger, increased podocyte permeability to albumin, induced dimerization of protein kinase G type I alpha (PKGIα), and activation of PKGIα‐dependent signaling. These data suggest the involvement of calcium and PKGIα signaling in insulin‐enhanced filtration barrier permeability in podocytes.