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NEDD8 ultimate buster‐1 regulates the abundance of TRF1 at telomeres by promoting its proteasomal degradation
Author(s) -
Jeong Yu Young,
Her Joonyoung,
Chung In Kwon
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12221
Subject(s) - nedd8 , telomere , microbiology and biotechnology , proteasome , degradation (telecommunications) , chemistry , signal transducing adaptor protein , telomere binding protein , telomerase , ubiquitin , biology , biochemistry , dna binding protein , signal transduction , dna , ubiquitin ligase , gene , transcription factor , telecommunications , computer science
The human telomeric protein TRF1 negatively regulates telomere length by inhibiting the access of telomerase to telomeres. Here, we describe a novel function of NEDD8 ultimate buster‐1 (NUB1) for regulating the levels of TRF1 at telomeres. NUB1 is a NEDD8‐interacting protein, which down‐regulates the NEDD8 conjugation system. We showed that NUB1 physically interacts with TRF1 and promotes its degradation by the proteasome in the absence of NEDD8 conjugation. We also demonstrated that TRF1 is conjugated to NEDD8, and that neddylated TRF1 is targeted to the proteasome for degradation in a NUB1‐dependent manner. These data suggest that NUB1 participates in telomere maintenance by regulating the levels of TRF1 at telomeres through both NEDD8‐dependent and NEDD8‐independent pathways.