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The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d‐ galacturonic acid from pectin
Author(s) -
Alazi Ebru,
Niu Jing,
Kowalczyk Joanna E.,
Peng Mao,
Aguilar Pontes Maria Victoria,
Kan Jan A. L.,
Visser Jaap,
Vries Ronald P.,
Ram Arthur F. J.
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12211
Subject(s) - aspergillus niger , pectin , biochemistry , cell wall , chemistry , activator (genetics) , catabolism , metabolism , monosaccharide , biology , gene
We identified the d‐ galacturonic acid (GA)‐responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger , which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome‐wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.