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Resting state of respiratory Complex I from Escherichia coli
Author(s) -
Belevich Nikolai,
Verkhovskaya Marina
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12199
Subject(s) - escherichia coli , chemistry , pyranine , biophysics , fluorescence , transition (genetics) , membrane , biochemistry , photochemistry , biology , physics , quantum mechanics , gene
Respiratory Complex I from Escherichia coli may exist in two states, resting (R) and active (A). The conversion from the R‐ to A‐forms occurs spontaneously upon turnover. The fast resting‐to‐active (R/A) transition of membrane‐bound and purified Complex I was studied with the stopped‐flow technique by following NADH oxidation either by absorption decay at 340 nm or using the fluorescent pH indicator, trisodium 8‐hydroxypyrene‐1,3,6‐trisulfonate (pyranine). The R/A transition of Complex I from E. coli occurs upon its turnover in a time interval of ~ 1.5 s. Comparisons between the bacterial Complex I R/A transition and the active/deactive transition of mitochondrial Complex I are discussed.