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Micro RNA ‐140‐5p impairs zebrafish embryonic bone development via targeting BMP ‐2
Author(s) -
Gan Shiquan,
Huang Zhaoqin,
Liu Ning,
Su Renxiang,
Xie Guie,
Zhong Beibei,
Zhang Kai,
Wang Shang,
Hu Xiang,
Zhang Jian,
Xiang Shuanglin
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12190
Subject(s) - zebrafish , microinjection , rna , bone morphogenetic protein , microbiology and biotechnology , embryo , morpholino , messenger rna , biology , bone morphogenetic protein 2 , embryonic stem cell , rna binding protein , gene , genetics , in vitro
Micro RNA ‐140‐5p (mi RNA ‐140‐5p) is important for embryonic bone development. In this study, we found that mi RNA ‐140‐5p and its binding site in the 3′ UTR of bone morphogenetic protein 2 ( BMP ‐2) are highly conserved among vertebrates, and mi RNA ‐140‐5p negatively regulates both zebrafish and human BMP ‐2 genes. Microinjection of mi RNA ‐140‐5p or BMP ‐2b morpholino into zebrafish embryos led to a similar phenotype, including shortened tails, curved trunks, and defects in cranial cartilage. Moreover, mi RNA ‐140‐5p injection induced zebrafish embryo malformation that could be significantly rescued by microinjection of BMP ‐2 mRNA . In conclusion, our results indicated that mi RNA ‐140‐5p regulates zebrafish embryonic bone development via targeting BMP ‐2.