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The RacGAP protein FilGAP is a negative regulator of chemokine‐promoted lymphocyte migration
Author(s) -
Iida Toru,
Saito Koji,
Katagiri Koko,
Kinashi Tatsuo,
Ohta Yasutaka
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12189
Subject(s) - microbiology and biotechnology , chemotaxis , gtpase , lymphocyte , regulator , chemokine , cell migration , chemistry , biology , immunology , cell , immune system , biochemistry , receptor , gene
Rho family small GTPases regulate lymphocyte migration induced by chemokines. However, how lymphocyte migration is regulated by Rho GTPases remains to be elucidated. Here, we identified FilGAP, a Rac‐specific GAP, as a negative regulator of lymphocyte polarization and migration. Depletion of FilGAP in mouse pro‐B BAF cells increased cellular elongation and membrane protrusion after stimulation of the cells with SDF‐1α, which caused increased migration speed. Although FilGAP is detectable both at the front and rear of polarized cells, FilGAP appears to be concentrated at the tip of retracting lamellae of moving lymphocytes. Moreover, depletion of FilGAP increased activation of Rac at the front of polarized cells. Thus, FilGAP may inhibit lamellae extension at the front of moving lymphocytes.