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Transient receptor potential A1 channels regulate epithelial cell barriers formed by MDCK cells
Author(s) -
Dembla Sandeep,
Hasan Nouma,
Becker Alexander,
Beck Andreas,
Philipp Stephan Ernst
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12183
Subject(s) - transient receptor potential channel , microbiology and biotechnology , chemistry , stimulation , biophysics , receptor , cell culture , biology , biochemistry , neuroscience , genetics
Transient receptor potential A1 channels are well‐known as chemosensors in neuronal cells. However, recent studies also point to non‐neuronal functions in epithelia. Here, we show that TRPA 1 channels are expressed in epithelial MDCK II cells and contribute to Ca 2+ influx and whole‐cell currents after stimulation with AITC . Stimulation of TRPA 1 channels induced an immediate reduction of the transepithelial resistance of MDCK II cell layers that was blocked by the TRPA 1 antagonist HC ‐030031. The present data provide strong evidence for a new role of TRPA 1 channels in regulating the tightness of epithelial cell barriers.