Premium
SAMM 50 Affects Mitochondrial Morphology through the Association of Drp1 in Mammalian Cells
Author(s) -
Liu Shuo,
Gao Yali,
Zhang Cheng,
Li Han,
Pan Shiyi,
Wang Xiaoli,
Du Shiming,
Deng Zixin,
Wang Lianrong,
Song Zhiyin,
Chen Shi
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12170
Subject(s) - morphology (biology) , association (psychology) , biology , microbiology and biotechnology , zoology , psychology , psychotherapist
Mitochondrial fission and fusion activities are important for cell survival and function. Drp1 is a GTP ase protein responsible for mitochondrial division, and SAMM 50 is responsible for protein sorting and assembly. We demonstrated that SAMM 50 overexpression results in Drp1‐dependent mitochondrial fragmentation in HeLa cells. However, the mitochondrial fragmentation induced by SAMM 50 overexpression could be reversed through co‐expression with MFN 2. Furthermore, SAMM 50 interacts with Drp1 both in vivo and in vitro . The mitochondria in SAMM 50 knockdown HeLa cells displayed a swollen phenotype, and the levels of the SAM complex and OPA 1, along with the mitochondrial Drp1 levels, significantly decreased. In addition, mitochondrial inheritance was impaired in SAMM 50 silenced cells. These results suggest that SAMM 50 affects the Drp1‐dependent mitochondrial morphology.