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Labeling of virus components for advanced, quantitative imaging analyses
Author(s) -
Sakin Volkan,
Paci Giulia,
Lemke Edward A.,
Müller Barbara
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12131
Subject(s) - computer science , exploit , computational biology , replication (statistics) , virus , biological system , virology , biology , computer security
In recent years, investigation of virus–cell interactions has moved from ensemble measurements to imaging analyses at the single‐particle level. Advanced fluorescence microscopy techniques provide single‐molecule sensitivity and subdiffraction spatial resolution, allowing observation of subviral details and individual replication events to obtain detailed quantitative information. To exploit the full potential of these techniques, virologists need to employ novel labeling strategies, taking into account specific constraints imposed by viruses, as well as unique requirements of microscopic methods. Here, we compare strengths and limitations of various labeling methods, exemplify virological questions that were successfully addressed, and discuss challenges and future potential of novel approaches in virus imaging.