Premium
Differentiation‐inducing factor 2 modulates chemotaxis via the histidine kinase DhkC‐dependent pathway in Dictyostelium discoideum
Author(s) -
Kuwayama Hidekazu,
Kubohara Yuzuru
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12111
Subject(s) - chemotaxis , dictyostelium discoideum , dictyostelium , microbiology and biotechnology , biology , histidine kinase , signal transduction , mycetozoa , mutant , biochemistry , receptor , gene
Differentiation‐inducing factor 1( DIF ‐1) and DIF ‐2 are signaling molecules that control chemotaxis in Dictyostelium discoideum . Whereas DIF ‐1 suppresses chemotaxis in shallow cAMP gradients, DIF ‐2 enhances chemotaxis under the same conditions via a phosphodiesterase, response regulator A (RegA), which is a part of the DhkC–RdeA–RegA two‐component signaling system. In this study, to investigate the mechanism of the chemotaxis regulation by DIF ‐2, we examined the effects of DIF ‐2 (and DIF ‐1) on chemotaxis in rdeA − and dhkC − mutant strains. In the parental wild‐type strains, chemotactic cell movement was suppressed with DIF ‐1 and enhanced with DIF ‐2 in shallow cAMP gradients. In contrast, in both rdeA − and dhkC − strains, chemotaxis was suppressed with DIF ‐1 but unaffected by DIF ‐2. The results suggest that DIF ‐2 modulates chemotaxis via the DhkC–RdeA–RegA signaling system.