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KLF 5 promotes cell migration by up‐regulating FYN in bladder cancer cells
Author(s) -
Du Chong,
Gao Yang,
Xu Shan,
Jia Jing,
Huang Zhixin,
Fan Jinhai,
Wang Xinyang,
He Dalin,
Guo Peng
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12069
Subject(s) - fyn , cell migration , lamellipodium , cancer research , cancer cell , microbiology and biotechnology , cell growth , chemistry , gene knockdown , lysophosphatidic acid , carcinogenesis , cell , phosphorylation , biology , cancer , biochemistry , proto oncogene tyrosine protein kinase src , receptor , apoptosis , genetics , gene
Krüppel‐like factor 5 ( KLF 5) promotes cell proliferation of bladder cancer. However, whether KLF 5 regulates other cell processes in bladder cancer is not clear. We found that KLF 5 increases cell migration and lamellipodia formation, expression of FYN and phosphorylation of FAK in bladder cancer cells. In addition, KLF 5 promotes transcription of FYN through binding to its promoter. FYN overexpression rescues cell migration and lamellipodia formation reduced by KLF 5 knockdown. Furthermore, the KLF 5/ FYN /p‐ FAK axis is necessary for lysophosphatidic acid (LPA) to promote cell migration. Our findings indicate that both KLF 5 and FYN are important in the regulation of cell migration in bladder cancer cells. We propose the KLF 5/ FYN /p‐ FAK axis as a potential therapeutic target in bladder cancer.