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Knockdown of Lmo7 inhibits chick myogenesis
Author(s) -
Possidonio Ana C. B.,
Soares Carolina P.,
Fontenele Marcio,
Morris Eduardo R.,
Mouly Vincent,
Costa Manoel L.,
Mermelstein Claudia
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12067
Subject(s) - myogenesis , gene knockdown , wnt3a , biology , wnt signaling pathway , myod , myocyte , microbiology and biotechnology , beta catenin , skeletal muscle , signal transduction , cell culture , anatomy , genetics
The multifunctional protein Lmo7 has been implicated in some aspects of myogenesis in mammals. Here we studied the distribution and expression of Lmo7 and the effects of Lmo7 knockdown in primary cultures of chick skeletal muscle cells. Lmo7 was localized within the nuclei of myoblasts and at the perinuclear region of myotubes. Knockdown of Lmo7 using si RNA specific to chick reduces the number and width of myotubes and the number of MyoD positive‐myoblasts. Both Wnt3a enriched medium and Bio, activators of the Wnt/beta‐catenin pathway, could rescue the effects of the Lmo7 knockdown suggesting a crosstalk between the Wnt/beta‐catenin and Lmo7‐mediated signaling pathways. Our data shows a role of Lmo7 during the initial events of chick skeletal myogenesis, particularly in myoblast survival.