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Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p
Author(s) -
Watanabe Yuichi,
Kawaguchi Kosuke,
Okuyama Naoki,
Sugawara Yuri,
Obita Takayuki,
Mizuguchi Mineyuki,
Morita Masashi,
Imanaka Tsuneo
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12044
Subject(s) - trypanosoma brucei , chemistry , biology , computational biology , biochemistry , gene
The interaction of Trypanosoma brucei ( Tb ) Pex5p and its receptor Tb Pex14p is essential for the translocation of newly synthesized matrix proteins into the glycosome. Here, we reveal that only the third WXXXF/Y motif of Tb Pex5p is involved in the interaction and that negative charge of the fourth amino acid is important. We suggest that Phe35 and Phe52 of Tb Pex14p interact with Trp318 and Phe322 in the third motif and that the Lys56 adjacent to Phe35/Phe52 associates with the fourth Glu in the motif to make the complex. This information is expected to be useful for developing anti‐trypanosomal drugs.