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Allelic variation in the vacuolar TPK 1 channel affects its calcium dependence and may impact on stomatal conductance
Author(s) -
Hartley Tom N.,
Maathuis Frans J. M.
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12035
Subject(s) - conductance , biophysics , allele , stomatal conductance , patch clamp , chemistry , cytoplasm , biology , mutant , gene , botany , biochemistry , photosynthesis , receptor , physics , condensed matter physics
Natural variation can be exploited to identify allelic variants of proteins. In this study, patch clamp was used to determine transport properties of two At TPK 1 alleles from Landsberg and Kas‐2 ecotypes. No difference in conductance or ion selectivity was observed but the Kas version of TPK 1 showed different Ca 2+ dependence in its open probability compared to Ler. Leaves from Kas showed lower rates of water loss than those of Ler, in either the absence or presence of ABA , an observation that is consistent with higher TPK 1 channel activity at comparable cytoplasmic Ca 2+ concentrations. A model that explains the results is presented.