Premium
Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression
Author(s) -
Cui Yazhou,
Luan Jing,
Li Haiying,
Zhou Xiaoyan,
Han Jinxiang
Publication year - 2016
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1002/1873-3468.12024
Subject(s) - microvesicles , microbiology and biotechnology , exosome , microrna , wnt signaling pathway , stromal cell , osteoblast , chemistry , cell , cell signaling , mesenchymal stem cell , signal transduction , biology , cancer research , biochemistry , gene , in vitro
Mineralizing osteoblasts (MOBs) can release exosomes, although the functional significance remains unclear. In the present study, we demonstrate that exosomes derived from mineralizing pre‐osteoblast MC3T3‐E1 cells can promote bone marrow stromal cell (ST2) differentiation to osteoblasts. We reveal that MOB‐derived exosomes significantly influence miRNA profiles in recipient ST2 cells, and these changes tend to activate the Wnt signaling pathway by inhibiting Axin1 expression and increasing β‐catenin expression. We also suggest that MOB derived‐exosomes partly induce the variation in miRNA expression in recipient ST2 cells by exosomal miRNA transfer. These findings suggest an exosome‐mediated mode of cell‐to‐cell communication in the osteogenic microenvironment, and also indicate the potential of MOB exosomes in bone tissue engineering.