Open AccessNovel Bioreactors for the ex vivo Cultivation of Hematopoietic Cells
Author(s) -
Jelinek N.,
Schmidt S.,
Hilbert U.,
Thoma S.,
Biselli M.,
Wandrey C.
Publication year - 2002
Publication title -
engineering in life sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.547
H-Index - 57
eISSN - 1618-2863
pISSN - 1618-0240
DOI - 10.1002/1618-2863(200201)2:1<15::aid-elsc15>3.0.co;2-5
Subject(s) - microcarrier , bioreactor , stromal cell , haematopoiesis , ex vivo , microbiology and biotechnology , cell culture , chemistry , biology , cell , stem cell , in vivo , biochemistry , cancer research , botany , genetics
One major requirement for a hematopoietic cell culture system is that cell cultivation be controlled. The control and regulation of the pO 2 and the pH value which greatly influence the differentiation of hematopoietic cells are of special importance. Two novel bioreactors are presented: a stirred system for the stroma‐free suspension culture and a fixed‐bed reactor for the immobilized coculture of stromal and hematopoietic cells on porous microcarriers. Both production systems have a process control. For comparative studies on a small scale miniaturized systems were developed.