Premium
Preparation of an Organized Film Composed of Polymers, Avidin, and Concanavalin A and Its Binding Properties
Author(s) -
Anzai Junichi,
Akase Sumiko
Publication year - 2002
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/1616-5195(200211)2:8<361::aid-mabi361>3.0.co;2-0
Subject(s) - avidin , concanavalin a , polymer , chemistry , cationic polymerization , thin film , polymer chemistry , chemical engineering , biotin , materials science , nanotechnology , organic chemistry , biochemistry , engineering , in vitro
The multilayer thin films composed of polymers, avidin, and concanavalin A (Con A) were prepared on the surface of a quartz slide by alternate deposition of anionic and cationic polymers and the proteins. The loading of avidin and Con A in the film was evaluated spectroscopically by using Texas Red tagged avidin and Con A. Avidin molecules assembled in the film caused its binding activity toward biotin and analogue 2‐(4′‐hydroxyphenylazo)benzoic acid to be retained. The polymer layers contributed to improving stability of the protein film.Polymer/Con A/avidin composite film on a solid surface.