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Self‐assembled plasmid DNA network prepared through both triple‐helix formation and streptavidin–biotin interaction
Author(s) -
Kawabata Yosuke,
Ooya Tooru,
Lee Won Kyu,
Yui Nobuhiko
Publication year - 2002
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/1616-5195(200206)2:5<195::aid-mabi195>3.0.co;2-i
Subject(s) - triple helix , plasmid , recombinant dna , dna supercoil , dna , helix (gastropod) , chemistry , oligonucleotide , streptavidin , biophysics , surface plasmon resonance , crystallography , nanotechnology , biotin , materials science , stereochemistry , biochemistry , biology , gene , dna replication , ecology , snail , nanoparticle
Plasmid DNA responds to external stimuli to change the degree of supercoiling. As a result of its unique topological change, plasmid DNA is considered to be a new functional material. In addition to its stimuli‐responsiveness, plasmid DNA has the capability of forming a triple helix with a triple‐helix‐forming oligonucleotide (TFO). The triple helix is formed in aqueous conditions and arises through recognition of specific sequences, and may lead to the formation of supramolecular architectures. With this in mind, we analyze the self‐assembly of recombinant plasmid DNA with two sequences for triple‐helix formation and TFO by means of surface plasmon resonance. In addition, the network formed by self‐assembly of the recombinant plasmid DNA with biotinated‐TFO and streptavidin is prepared and evaluated by atomic force microscopy (AFM).Schematic illustration of plasmid DNA network.