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Relative Lectin Binding Properties of T‐Antigen‐Containing Glycopolymers: Copolymerization of N ‐Acryloylated T‐Antigen Monomer vs. Graft Conjugation of Aminated T‐Antigen Ligands onto Poly( N ‐acryloxysuccinimide)
Author(s) -
Baek MyungGi,
Roy René
Publication year - 2001
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/1616-5195(20011001)1:7<305::aid-mabi305>3.0.co;2-b
Subject(s) - antigenicity , chemistry , monomer , lectin , antigen , nanocellulose , glycoconjugate , copolymer , biochemistry , polymer chemistry , organic chemistry , cellulose , biology , immunology , polymer
Water‐soluble T‐antigen‐ (Galβ(1–3)‐GalNAcα) containing random glycopolymers were synthesized by two strategies: i) radical copolymerization of N ‐acryloylated monomers with (NH 4 ) 2 S 2 O 8 and ii) graft conjugation of an end‐group‐aminated T‐antigen together with labeling reagent ( 16 ) by amidation onto poly( N ‐acryloxysuccinimide) and its derivatives followed by quenching with NH 4 OH. All glycoconjugates demonstrated antigenicity by double‐radial immunodiffusion assays with peanut lectin from Arachis hypogaea . The biocytin‐labeled terpolymer ( 23 ) also showed practical heterobifunctional antigenicity toward peanut lectin and streptavidin, giving the corresponding two precipitin bands in the assay.