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Entrapment of α ‐Chymotrypsin into Hollow Polyelectrolyte Microcapsules
Author(s) -
Tiourina Olga P.,
Antipov Alexei A.,
Sukhorukov Gleb B.,
Larionova Natalia I.,
Lvov Yuri,
Möhwald Helmuth
Publication year - 2001
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/1616-5195(20010701)1:5<209::aid-mabi209>3.0.co;2-y
Subject(s) - polyelectrolyte , chemistry , chemical engineering , chymotrypsin , allylamine , polymer chemistry , trypsin , organic chemistry , polymer , enzyme , engineering
Stable hollow polyelectrolyte capsules were produced by the layer‐by‐layer assembling of non‐biodegradable polyelectrolytes – poly(allylamine) and poly(styrenesulfonate) on melamine formaldehyde microcores followed by the core decomposition at low pH. A proteolytic enzyme, α ‐chymotrypsin, was encapsulated into these microcapsules with high yields of up to 100%. The encapsulation procedure was based on the protein adsorption onto the capsule shells and on the pH‐dependent opening and closing of capsule wall pores. The protein in the capsules retained a high activity, and thermo‐ and storage stability. The nanostructured polyelectrolyte shell protected the proteinase from a high molecular weight inhibitor. Such enzyme‐loaded capsules can be used as microreactors for biocatalysis.