Premium
Poly ( L ‐lactide)‐Degrading Enzyme Produced by Amycolatopsis sp.
Author(s) -
Pranamuda Hardaning,
Tsuchii Akio,
Tokiwa Yutaka
Publication year - 2001
Publication title -
macromolecular bioscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.924
H-Index - 105
eISSN - 1616-5195
pISSN - 1616-5187
DOI - 10.1002/1616-5195(200101)1:1<25::aid-mabi25>3.0.co;2-3
Subject(s) - enzyme , polyester , casein , chemistry , silk , size exclusion chromatography , molecular mass , polymer chemistry , biochemistry , materials science , organic chemistry , composite material
Poly ( L ‐lactide) (PLLA)‐degrading enzyme was produced in a liquid culture of Amycolatopsis sp. (strain 41). In comparison with polyester substrates, silk powder from silkworm cocoons was the most effective in inducing enzyme production within 5 d. Application to DEAE and Superdex 75 columns resulted in a major protein with molecular weight estimated to be 42 kDa from size exclusion chromatography or 40 kDa from SDS‐PAGE analysis. Optimum pH and temperature are 6.0 and 37–45°C, respectively. Besides PLLA, the enzyme degrades casein, silk powder and Suc‐(Ala) 3 ‐ p NA at an even lower level than Proteinase‐K, but not Suc‐(Gly) 3 ‐ p NA, poly ( ε ‐caprolactone) and poly ( β ‐hydroxybutyrate).