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Surface–Plasmon Fluorescence Spectroscopy
Author(s) -
Neumann T.,
Johansson M.L.,
Kambhampati D.,
Knoll W.
Publication year - 2002
Publication title -
advanced functional materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.069
H-Index - 322
eISSN - 1616-3028
pISSN - 1616-301X
DOI - 10.1002/1616-3028(20020916)12:9<575::aid-adfm575>3.0.co;2-4
Subject(s) - materials science , fluorophore , biosensor , surface plasmon , fluorescence , plasmon , chromophore , excited state , nanotechnology , resonant inductive coupling , energy transfer , optoelectronics , photochemistry , chemical physics , optics , atomic physics , chemistry , physics
We summarize some features of the recently introduced surface–plasmon field‐enhanced fluorescence spectroscopy (SPFS): a novel technique offering an increased sensitivity for monitoring interfacial binding reactions in biosensor formats. We briefly discuss the enhancement factors obtainable at resonant excitation of surface–plasmon modes propagating along a (noble) metal/dielectric interface and refer to the (Förster) energy transfer mechanisms operating for chromophores excited near metal surfaces. As a first example, we present data obtained during the binding of fluorophore‐doped latex particles to a functionalized interface. Then, experiments are described with surface‐attached oligonucleotide catcher probes and complementary target strands from solution, demonstrating the potential of SPFS for monitoring hybridization reactions.