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Organic disulfides as a means to generate streak‐free two‐dimensional maps with narrow range basic immobilized pH gradient strips as first dimension
Author(s) -
Olsson Ingmar,
Larsson Kjell,
Palmgren Ronnie,
Bjellqvist Bengt
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200211)2:11<1630::aid-prot1630>3.0.co;2-n
Subject(s) - streaking , streak , dimension (graph theory) , strips , immobilized ph gradient , disulfide bond , reproducibility , chromatography , range (aeronautics) , chemistry , critical dimension , analytical chemistry (journal) , materials science , optics , computer science , artificial intelligence , physics , mineralogy , biochemistry , mathematics , isoelectric focusing , composite material , pure mathematics , enzyme
Streaking is a severe problem when narrow range basic immobilized pH gradient strips are used as the first dimension of two‐dimensional (2‐D) electrophoresis. It is demonstrated that this cysteinyl related streaking is eliminated when focusing is done in the presence of hydroxyethyl disulfide (DeStreak™). Use of DeStreak also results in 2‐D maps with simplified spot patterns and improved reproducibility.