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Renal cell carcinoma: Handling and treatment
Author(s) -
Sarto Cecilia,
Valsecchi Cristina,
Mocarelli Paolo
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200211)2:11<1627::aid-prot1627>3.0.co;2-k
Subject(s) - renal cell carcinoma , reproducibility , proteomics , chromatography , carcinoma , gel electrophoresis , cell , biology , chemistry , microbiology and biotechnology , pathology , medicine , biochemistry , gene
The quality of samples and of pre‐analytical steps are crucial in all biological tests, this is dramatically true in proteomics analysis. In renal cell carcinoma preparation for two‐dimensional gel electrophoresis the time elapsed between sample collection and treatment, and the heterogeneity of tissues are considered in order to obtain high quality and reproducibility of spots. The mechanical dissection and cell separation by magnetic beads coated with anti‐Ber and EP4 antibodies to minimize the contamination of nonepithelial cells are described.