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Establishing two‐dimensional gels for the analysis of Leishmania proteomes
Author(s) -
Acestor Nathalie,
Masina Slavica,
Walker John,
Saravia Nancy G.,
Fasel Nicolas,
Quadroni Manfredo
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200207)2:7<877::aid-prot877>3.0.co;2-d
Subject(s) - proteome , leishmania , chromatography , reproducibility , sample preparation , proteomics , solubilization , leishmania major , electrophoresis , chemistry , biology , computational biology , biochemistry , parasite hosting , computer science , world wide web , gene
Several different sample preparation methods for two‐dimensional electrophoresis (2‐DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P‐40 as detergent, which was simple to follow, and which produced 2‐DE gels of high resolution and reproducibility.