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Immunogenic proteins of Helicobacter pullorum , Helicobacter bilis and Helicobacter hepaticus identified by two‐dimensional gel electrophoresis and immunoblotting
Author(s) -
Kornilovs’ka Iryna,
Nilsson Ingrid,
Utt Meeme,
Ljungh Åsa,
Wadström Torkel
Publication year - 2002
Publication title -
proteomics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.26
H-Index - 167
eISSN - 1615-9861
pISSN - 1615-9853
DOI - 10.1002/1615-9861(200206)2:6<775::aid-prot775>3.0.co;2-r
Subject(s) - helicobacter , biology , microbiology and biotechnology , helicobacter pylori , fastidious organism , serology , gel electrophoresis , virulence , antiserum , bacteria , virology , antibody , immunology , biochemistry , gene , genetics
The ecological niches occupied by various species of Helicobacter are not yet known and the full spectrum of diseases associated with Helicobacter infections are not yet defined. Since these fastidious microaerofilic bacteria require special growth conditions new and improved molecular and serologic diagnostic methods have been developed to increase our understanding of their pathogenesis and virulence characteristics. Immunogenic cell surface proteins of Helicobacter pullorum, Helicobacter bilis , and Helicobacter hepaticus were characterised by proteomic techniques using two‐dimensional electrophoresis and immunoblotting with antisera from immunised rabbits. Cross‐reactivity between the three Helicobacter species were analysed after a four‐step cross‐absorption experiment. For H. pullorum, H. bilis and H. hepaticus 21, 13 and 27 specific immunogenic proteins, respectively, were identified. These proteins could be of important sero‐diagnostic value for analyses of sera from humans, laboratory animals and for the veterinarian field.